Generator

Part:BBa_K1486009:Design

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-27)


CxpR & Split IFP1.4 [Nterm + Nterm]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1481
    Illegal PstI site found at 2714
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 1481
    Illegal PstI site found at 2714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2925
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1718
    Illegal XhoI site found at 3085
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1481
    Illegal PstI site found at 2714
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1481
    Illegal PstI site found at 2714
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2129
    Illegal AgeI site found at 3496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

It is to be noted that this construct makes use of an Arabinose-inducible promoter, a standard Elowitz RBS, as well as a flexible linker 2 x (Gly-Gly-Gly-Gly-Ser) between CpxR and the two split parts of the IFP WARNING: It is also to be noted that basic parts are submitted with a start codon and a stop codon. To make a composite part, START and STOP codons of the basics parts may have been removed, as we only want a START codon at the beginning of a cds and a STOP codon at the end of a cds. You should consider it when looking at the part sequence.

Source

We received Michnick lab IFP1 and IFP2 sequences from [http://www.addgene.org/52899/ addgene].

References

Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). [http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2934.html An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.] Nature Methods, 6-6.